Image Measurements on ceiling mounted cooling systems
Image Helium extraction from natural gas
Image Solar Cooling
Image State of system and failure analyses
Image Thermostatic Expansion Valves
Image Prüflabor Wärmepumpen at ILK Dresden
Image Membrane-based Air Conditioning
Image Components for Cold Storage Systems
Image Thermal engines
Image Low noise and non metallic liquid-helium cryostat
Image Low temperature – test facilities
Image Test procedures for electrical components
Image Low Temperature Measuring Service
Image Calibration of Low Temperature Sensors
Image Cryostats, Non-Metallic and Metallic
Image Software modules

You are here:   /  Home


Research on cell-based spheroids

Federal Ministry of Economic Affairs and Energy (EURONORM)

02/2017-07/2019

Dr. rer. nat. Gabriele Spörl

+49-351-4081-604

Is cryobanking possible?

Motivation

  • Widespread-diseases: e.g. diabetes, liver and muscuskeletal diseases
    • Affected organs: pancreas, liver and e.g. cartialge tissue
  • Spheroid forming cells: Cartilage, skin, vessel, bone, tendon, heart, liver, pancreas and pulp cells
  • Suitability of cell-based spheroids
    • As regenerative therapeutics
    • For screening processes for the implementation of the REACH regulation (REACH: Registration, Evaluation, Authorisation and Restriction of Chemicals) on 3D tissue models
    • As a substitute for animal testing in toxicity tests
    • For drug testing in cancer research

Objective of the project

  • Stable and universally applicable model systems and specific spheroid systems
  • Novel non-toxic cryoprotective agents for various methods of cryogenic preservation
  • Basic knowledge of specific reractions of spheroids from cartilage and pulp cells (model tissues) to the effects of low temperatures (−80 °C up to −180 °C)
    → Requirement for basic tests on penetration behavior and the development of cryoprotocols
  • Basic knowledge of the penetration capability of the spheroid membrane for cryoprotectives
  • Cryoprotocols for various methods of cryogenic preservation for model systems and specific spheroid systems
  • Possibility of GMP-compliant (GMP: Good Manufacturing Practice) execution of cell or spheriod culture protocols and cryoprotocols

Project flow

Innovation

  • Creation and establishment of stable and universally applicable model systems and specific spheroid systems for systematic studies of the possibility of cryogenic preservation similarly built artificial and natural 3D tissues
  • Development of novel non-toxic cryoprotective agents and examination of their penetration behavior as moderators of the ice formation
  • Development of novel cryoprotocols for 3D tissues (spheroids)
  • Establishment of quantitatively evaluated detection methods by linking known processes in a novel way

Your Request

Further Projects

Image

Thermische Kälteerzeugung / Absorptionskältetechnik

Kraft-Wärme-Kälte-Kopplung, Fernwärme, Solarthermie oder Abwärme zur Kälteerzeugung

Image

Entwicklung eines schnellen Rechenverfahrens..

..für die Auslegung von Turbomaschinen basierend auf IBM

Image

Schalldämpfer mit integrierten Abgaswärmeübertrager

zur Verbesserung des Kaltstartverhaltens


Contact

Institut für Luft- und Kältetechnik - Gemeinnützige Gesellschaft mbH
Bertolt-Brecht-Allee 20, 01309 Dresden


Secretary to the Management

+49-351-4081-520

+49-351-4081-525

Image ISO 9001
Bild Zuse Mitglied Bild SIG